Association of the APC Gene Product with β-Catenin
B Rubinfeld, B Souza, I Albert, O Müller… - Science, 1993 - science.org
B Rubinfeld, B Souza, I Albert, O Müller, SH Chamberlain, FR Masiarz, S Munemitsu…
Science, 1993•science.orgMutations in the human APC gene are linked to familial adenomatous polyposis and to the
progression of sporadic colorectal and gastric tumors. To gain insight into APC function,
APC-associated proteins were identified by immunoprecipitation experiments. Antibodies to
APC precipitated a 95-kilodalton protein that was purified and identified by sequencing as β-
catenin, a protein that binds to the cell adhesion molecule E-cadherin. An antibody specific
to β-catenin also recognized the 95-kilodalton protein in the immunoprecipitates. These …
progression of sporadic colorectal and gastric tumors. To gain insight into APC function,
APC-associated proteins were identified by immunoprecipitation experiments. Antibodies to
APC precipitated a 95-kilodalton protein that was purified and identified by sequencing as β-
catenin, a protein that binds to the cell adhesion molecule E-cadherin. An antibody specific
to β-catenin also recognized the 95-kilodalton protein in the immunoprecipitates. These …
Mutations in the human APC gene are linked to familial adenomatous polyposis and to the progression of sporadic colorectal and gastric tumors. To gain insight into APC function, APC-associated proteins were identified by immunoprecipitation experiments. Antibodies to APC precipitated a 95-kilodalton protein that was purified and identified by sequencing as β-catenin, a protein that binds to the cell adhesion molecule E-cadherin. An antibody specific to β-catenin also recognized the 95-kilodalton protein in the immunoprecipitates. These results suggest that APC is involved in cell adhesion.
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