[HTML][HTML] Mitochondrial lysine uptake limits hepatic lysine oxidation in rats fed diets containing 5, 20 or 60% casein
KP Blemings, TD Crenshaw, NJ Benevenga - The Journal of nutrition, 1998 - Elsevier
Abstract Sixty male Sprague-Dawley rats were randomly allotted to receive diets containing
5, 20 or 60% casein. Rats had access to the diet only during the initial 8 h of the daily 12-h
dark period. Hepatic mitochondrial lysine uptake, lysine α-ketoglutarate reductase (LKR)
and saccharopine dehydrogenase (SacD) activities, and in vitro lysine oxidation (LOX) were
measured 0, 6, 12, 18 and 24 h after the start of the dark period. Diurnal variation of
mitochondrial lysine uptake was not detected (P> 0.10) although uptake varied 3-fold over …
5, 20 or 60% casein. Rats had access to the diet only during the initial 8 h of the daily 12-h
dark period. Hepatic mitochondrial lysine uptake, lysine α-ketoglutarate reductase (LKR)
and saccharopine dehydrogenase (SacD) activities, and in vitro lysine oxidation (LOX) were
measured 0, 6, 12, 18 and 24 h after the start of the dark period. Diurnal variation of
mitochondrial lysine uptake was not detected (P> 0.10) although uptake varied 3-fold over …
