Characterization of a sodium-dependent vitamin transporter mediating the uptake of pantothenate, biotin and lipoate in human placental choriocarcinoma cells
The characteristics of the uptake of the vitamin pantothenate into JAr human placental
choriocarcinoma cells were investigated and these cells were found to accumulate the
vitamin against a concentration gradient by a Na+-dependent process. Substitution of Na+
with other monovalent cations abolished the uptake completely. The transport process
showed no preference for any particular anion. Kinetic analysis indicated the presence of a
single saturable transport system with a Michaelis-Menten constant of 2.1±0.2 μ and a …
choriocarcinoma cells were investigated and these cells were found to accumulate the
vitamin against a concentration gradient by a Na+-dependent process. Substitution of Na+
with other monovalent cations abolished the uptake completely. The transport process
showed no preference for any particular anion. Kinetic analysis indicated the presence of a
single saturable transport system with a Michaelis-Menten constant of 2.1±0.2 μ and a …
