Reconstitution of lactic dehydrogenase. Noncovalent aggregation vs. reactivation. 1. Physical properties and kinetics of aggregation
G Zettlmeissl, R Rudolph, R Jaenicke - Biochemistry, 1979 - ACS Publications
G Zettlmeissl, R Rudolph, R Jaenicke
Biochemistry, 1979•ACS PublicationsMaterials and Methods Lactic dehydrogenase (LDH-M4) 1 from pig muscle and NADH were
obtained from Boehringer Mannheim GmbH (Mannheim); dithioerythritol was from
Calbiochem (Luzern). All other reagents were A-grade substances from Merck 1
Abbreviations used: LDH-M4, porcine lactic dehydrogenase from skeletal muscle; DTE,
dithioerythritol; NAD, nicotinamide adenosine dinucleotide; c and c0, concentration of
enzyme and guanidine hydrochloride, respectively; i5 „denatured state; A, aggregate; D …
obtained from Boehringer Mannheim GmbH (Mannheim); dithioerythritol was from
Calbiochem (Luzern). All other reagents were A-grade substances from Merck 1
Abbreviations used: LDH-M4, porcine lactic dehydrogenase from skeletal muscle; DTE,
dithioerythritol; NAD, nicotinamide adenosine dinucleotide; c and c0, concentration of
enzyme and guanidine hydrochloride, respectively; i5 „denatured state; A, aggregate; D …
Materials and Methods
Lactic dehydrogenase (LDH-M4) 1 from pig muscle and NADH were obtained from Boehringer Mannheim GmbH (Mannheim); dithioerythritol was from Calbiochem (Luzern). All other reagents were A-grade substances from Merck
1 Abbreviations used: LDH-M4, porcine lactic dehydrogenase from skeletal muscle; DTE, dithioerythritol; NAD, nicotinamide adenosine dinucleotide; c and c0, concentration of enzyme and guanidine hydrochloride, respectively; i5 „denatured state; A, aggregate; D, monomeric intermediate state; N, tetrameric native state; x, average degree of ag-gregation.
ACS Publications