Defects of the NADH dehydrogenase complex are predominantly manifested in
mitochondrial diseases and are significantly associated with the development of many late
onset neurological disorders such as Parkinson's disease. Here we describe an
immunocapture procedure for isolating this multisubunit membrane-bound complex from
human tissue. Using small amounts of immunoisolated protein, one-dimensional and two-
dimensional gel electrophoresis, matrix-assisted laser desorption ionization time-of-flight …