Formation of the catecholamine release-inhibitory peptide catestatin from chromogranin A: determination of proteolytic cleavage sites in hormone storage granules
CV Taylor, L Taupenot, SK Mahata, M Mahata… - Journal of Biological …, 2000 - ASBMB
The catestatin fragment of chromogranin A is an inhibitor of catecholamine release, but its
occurrence in vivo has not yet been verified, nor have its precise cleavage sites been
established. Here we found extensive processing of catestatin in chromogranin A, as judged
by catestatin radioimmunoassay of size-fractionated chromaffin granules. On mass
spectrometry, a major catestatin form was bovine chromogranin A 332–364; identity of the
peptide was confirmed by diagnostic Met 346 oxidation. Further analysis revealed two …
occurrence in vivo has not yet been verified, nor have its precise cleavage sites been
established. Here we found extensive processing of catestatin in chromogranin A, as judged
by catestatin radioimmunoassay of size-fractionated chromaffin granules. On mass
spectrometry, a major catestatin form was bovine chromogranin A 332–364; identity of the
peptide was confirmed by diagnostic Met 346 oxidation. Further analysis revealed two …
