Molecular analysis of a novel winged helix protein, WIN: expression pattern, DNA binding property, and alternative splicing within the DNA binding domain
KM Yao, M Sha, Z Lu, GG Wong - Journal of Biological Chemistry, 1997 - ASBMB
We have cloned a novel winged helix factor, WIN, from the rat insulinoma cell line, INS-1.
Northern blot analysis demonstrated that WIN is highly expressed in a variety of insulinoma
cell lines and rat embryonic pancreas and liver. In adults, WIN expression was detected in
thymus, testis, lung, and several intestinal regions. We determined the DNA sequences
bound in vitro by baculovirus-expressed WIN protein in a polymerase chain reaction-based
selection procedure. WIN was found to bind with high affinity to the selected sequence 5 …
Northern blot analysis demonstrated that WIN is highly expressed in a variety of insulinoma
cell lines and rat embryonic pancreas and liver. In adults, WIN expression was detected in
thymus, testis, lung, and several intestinal regions. We determined the DNA sequences
bound in vitro by baculovirus-expressed WIN protein in a polymerase chain reaction-based
selection procedure. WIN was found to bind with high affinity to the selected sequence 5 …
