Cyclooxygenase metabolism of endogenous arachidonic acid by cultured human tracheal epithelial cells
L Churchill, FH Chilton, JH Resau, R Bascom… - Am Rev Respir …, 1989 - atsjournals.org
L Churchill, FH Chilton, JH Resau, R Bascom, WC Hubbard, D Proud
Am Rev Respir Dis, 1989•atsjournals.orgThe epithelial cell may contribute to the regulation of piJlmonary function during
inflammatory diseases of the airways by producing metabolites of arachidonic acid (AA). We
have used human tracheal epithelial cells (HTE), grown in serum-free medium, to examine
cyclooxygenase metabolism of endogenous AA by these cells. Gas chromatography-
negative ion mass spectrometry demonstrated that, regardless of stimulus (buffer,
bradykinin, or the calcium ionophore A23187), epithelial cells produce PGE, and PGF211 …
inflammatory diseases of the airways by producing metabolites of arachidonic acid (AA). We
have used human tracheal epithelial cells (HTE), grown in serum-free medium, to examine
cyclooxygenase metabolism of endogenous AA by these cells. Gas chromatography-
negative ion mass spectrometry demonstrated that, regardless of stimulus (buffer,
bradykinin, or the calcium ionophore A23187), epithelial cells produce PGE, and PGF211 …
Summary
The epithelial cell may contribute to the regulation of piJlmonary function during inflammatory diseases of the airways by producing metabolites of arachidonic acid (AA). We have used human tracheal epithelial cells (HTE), grown in serum-free medium, to examine cyclooxygenase metabolism of endogenous AA by these cells. Gas chromatography-negative ion mass spectrometry demonstrated that, regardless of stimulus (buffer, bradykinin, or the calcium ionophore A23187), epithelial cells produce PGE, and PGF211 but no detectable levels of PGD" thromboxane 8" 6-keto-
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