Although Cystic fibrosis transmembrane conductance regulator (CFTR) has been shown to
regulate the activity of NHE3, the potential reciprocal interaction of NHE3 to modulate the
protein kinase A (PKA)-dependent regulation of CFTR in epithelial cells is still unknown. In
the present work, we describe experiments to define the interactions between CFTR and
NHE3 with the regulatory, scaffolding protein, NHERF that organize their PKA-dependent
regulation in a renal epithelial cell line that expresses endogenous CFTR. The expression of …