[HTML][HTML] Staphylococcus aureus - induced tumor necrosis factor - related apoptosis - inducing ligand expression mediates apoptosis and caspase-8 activation in …
EH Alexander, FA Rivera, I Marriott, J Anguita, KL Bost… - BMC microbiology, 2003 - Springer
EH Alexander, FA Rivera, I Marriott, J Anguita, KL Bost, MC Hudson
BMC microbiology, 2003•SpringerBackground Staphylococcus aureus infection of normal osteoblasts induces expression of
tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). Results Normal osteoblasts
were incubated in the presence of purified bacterial products over a range of concentrations.
Results demonstrate that purified surface structures and a selected superantigen present in
the extracellular environment are not capable of inducing TRAIL expression by osteoblasts.
Osteoblasts were co-cultured with S. aureus at various multiplicities of infection utilizing cell …
tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). Results Normal osteoblasts
were incubated in the presence of purified bacterial products over a range of concentrations.
Results demonstrate that purified surface structures and a selected superantigen present in
the extracellular environment are not capable of inducing TRAIL expression by osteoblasts.
Osteoblasts were co-cultured with S. aureus at various multiplicities of infection utilizing cell …
Background
Staphylococcus aureus infection of normal osteoblasts induces expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL).
Results
Normal osteoblasts were incubated in the presence of purified bacterial products over a range of concentrations. Results demonstrate that purified surface structures and a selected superantigen present in the extracellular environment are not capable of inducing TRAIL expression by osteoblasts. Osteoblasts were co-cultured with S. aureus at various multiplicities of infection utilizing cell culture chamber inserts. Results of those experiments suggest that direct contact between bacteria and osteoblasts is necessary for optimal TRAIL induction. Finally, S. aureus infection of osteoblasts in the presence of anti-TRAIL antibody demonstrates that TRAIL mediates caspase-8 activation and apoptosis of infected cells.
Conclusions
Collectively, these findings suggest a mechanism whereby S. aureus mediates bone destruction via induction of osteoblast apoptosis.
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