[HTML][HTML] p53-dependent caspase-2 activation in mitochondrial release of apoptosis-inducing factor and its role in renal tubular epithelial cell injury
R Seth, C Yang, V Kaushal, SV Shah… - Journal of Biological …, 2005 - ASBMB
We demonstrate the role of p53-mediated caspase-2 activation in the mitochondrial release
of apoptosis-inducing factor (AIF) in cisplatin-treated renal tubular epithelial cells. Gene
silencing of AIF with its small interfering RNA (siRNA) suppressed cisplatin-induced AIF
expression and provided a marked protection against cell death. Subcellular fractionation
and immunofluorescence studies revealed cisplatin-induced translocation of AIF from the
mitochondria to the nuclei. Pancaspase inhibitor benzyloxycarbonyl-Val-Ala-Asp …
of apoptosis-inducing factor (AIF) in cisplatin-treated renal tubular epithelial cells. Gene
silencing of AIF with its small interfering RNA (siRNA) suppressed cisplatin-induced AIF
expression and provided a marked protection against cell death. Subcellular fractionation
and immunofluorescence studies revealed cisplatin-induced translocation of AIF from the
mitochondria to the nuclei. Pancaspase inhibitor benzyloxycarbonyl-Val-Ala-Asp …