Concurrent detection of secreted products from human lymphocytes by microengraving: Cytokines and antigen-reactive antibodies
EM Bradshaw, SC Kent, V Tripuraneni, T Orban… - Clinical …, 2008 - Elsevier
Cell surface determinants, cytokines and antibodies secreted by hematopoietic cells are
used to classify their lineage and function. Currently available techniques are unable to
elucidate multiple secreted proteins while also assigning phenotypic surface-displayed
markers to the individual living cells. Here, a soft lithographic method, microengraving, was
adapted for the multiplexed interrogation of populations of individual human peripheral
blood mononuclear cells for secreted cytokines (IFN-γ and IL-6), antigen-specific antibodies …
used to classify their lineage and function. Currently available techniques are unable to
elucidate multiple secreted proteins while also assigning phenotypic surface-displayed
markers to the individual living cells. Here, a soft lithographic method, microengraving, was
adapted for the multiplexed interrogation of populations of individual human peripheral
blood mononuclear cells for secreted cytokines (IFN-γ and IL-6), antigen-specific antibodies …
