Among human natural killer (NK) cell intermediates in secondary lymphoid tissue (SLT), stage 3 CD34⁻CD117⁺CD161⁺CD94⁻ immature NK (iNK) cells uniquely express aryl hydrocarbon receptor (AHR) and interleukin-22 (IL-22), supporting a role in mucosal immunity. The mechanisms controlling proliferation and differentiation of these cells are unknown. Here we demonstrate that the IL-1 receptor IL-1R1 was selectively expressed by a subpopulation of iNK cells that localized proximal to IL-1β-producing conventional dendritic cells (cDCs) within SLT. IL-1R1^hi iNK cells required continuous exposure to IL-1β to retain AHR and IL-22 expression, and they proliferate in direct response to cDC-derived IL-15 and IL-1β. In the absence of IL-1β, a substantially greater fraction of IL-1R1^hi iNK cells differentiated to stage 4 NK cells and acquired the ability to kill and secrete IFN-γ. Thus, cDC-derived IL-1β preserves and expands IL-1R1^hiIL-22⁺AHR⁺ iNK cells, potentially influencing human mucosal innate immunity during infection.