Replication sites as revealed by double label immunofluorescence against proliferating cell nuclear antigen (PCNA) and bromodeoxyuridine (BrdU) in synchronized …
H Takanari, H Yamanaka, H Mitani, K Izutsu - Biology of the Cell, 1994 - Elsevier
Double label immunofluorescence against PCNA and BrdU clearly revealed several
characteristics of DNA replication sites in synchronized CHO cells. We observed that the
distribution of replication sites changed from early to late S phase, particularly in the
nucleolar and perinuclear regions, and the amount of PCNA in each replication site
markedly decreased or disappeared with the progression of S phase. Although co-
localization of PCNA and BrdU was usually seen, the intensity of fluorescence occasionally …
characteristics of DNA replication sites in synchronized CHO cells. We observed that the
distribution of replication sites changed from early to late S phase, particularly in the
nucleolar and perinuclear regions, and the amount of PCNA in each replication site
markedly decreased or disappeared with the progression of S phase. Although co-
localization of PCNA and BrdU was usually seen, the intensity of fluorescence occasionally …