Purification and characterization of human coagulation factor V.
WH Kane, PW Majerus - Journal of Biological Chemistry, 1981 - ASBMB
We have purified human coagulation Factor V 6,000-fold to homogeneity from citrated
plasma using polyethylene glycol 6000 precipitation, adsorption of Factor V to barium citrate,
DEAE-Sepharose chromatography, and gel filtration on Ultrogel AcA 34 (yield 21%). Human
Factor V is a single polypeptide chain before and after disulfide bond reduction with an
apparent Mr= 335,000 as determined by electrophoresis on 5% acrylamide sodium dodecyl
sulfate gels. Human Factor V is a glycoprotein containing 13% of weight carbohydrate and …
plasma using polyethylene glycol 6000 precipitation, adsorption of Factor V to barium citrate,
DEAE-Sepharose chromatography, and gel filtration on Ultrogel AcA 34 (yield 21%). Human
Factor V is a single polypeptide chain before and after disulfide bond reduction with an
apparent Mr= 335,000 as determined by electrophoresis on 5% acrylamide sodium dodecyl
sulfate gels. Human Factor V is a glycoprotein containing 13% of weight carbohydrate and …
