Interaction of a 59-kDa connective tissue matrix protein with collagen I and collagen II
E Hedbom, D Heinegård - Journal of Biological Chemistry, 1989 - ASBMB
We have studied binding to collagen of the 59-kDa protein present in most connective
tissues. Collagen fibril formation, measured as increasing turbidity, was markedly retarded
and reduced by the presence of small amounts of this protein. This was true for both
collagen I and collagen II. The effect was also observed when pepsin-treated collagens
were used, indicating that interaction with the telopeptides is not involved. The proportion of
collagen precipitated in the assay was not or only marginally reduced. Thus, the altered …
tissues. Collagen fibril formation, measured as increasing turbidity, was markedly retarded
and reduced by the presence of small amounts of this protein. This was true for both
collagen I and collagen II. The effect was also observed when pepsin-treated collagens
were used, indicating that interaction with the telopeptides is not involved. The proportion of
collagen precipitated in the assay was not or only marginally reduced. Thus, the altered …