Angiogenic synergy of bFGF and VEGF is antagonized by Angiopoietin-2 in a modified in vivo Matrigel assay
CD Ley, MWB Olsen, EL Lund, PEG Kristjansen - Microvascular research, 2004 - Elsevier
A murine modification of the Matrigel chamber assay originally developed for use on rats is
presented. This modified assay permits improved quantification due to subcutaneous
Matrigel implants of constant shape and volume. We have quantitatively assessed the
angiogenic potential of the growth factors basic fibroblast growth factor (bFGF), VEGF, and
Angiopoietin-2 (Ang-2) with special emphasis on their mutual interactions. A reproducible
dose–response relationship for bFGF was established for doses between 150 and 1000 ng …
presented. This modified assay permits improved quantification due to subcutaneous
Matrigel implants of constant shape and volume. We have quantitatively assessed the
angiogenic potential of the growth factors basic fibroblast growth factor (bFGF), VEGF, and
Angiopoietin-2 (Ang-2) with special emphasis on their mutual interactions. A reproducible
dose–response relationship for bFGF was established for doses between 150 and 1000 ng …