Endogenous apurinic/apyrimidinic sites in genomic DNA of mammalian tissues

J Nakamura, JA Swenberg - Cancer research, 1999 - AACR
J Nakamura, JA Swenberg
Cancer research, 1999AACR
Apurinic/apyrimidinic (AP) sites are one of the most frequent lesions in DNA. Using a highly
sensitive slot blot assay, we determined the number and condition of endogenous AP sites
in normal tissues of rats and human liver. The number of AP sites (50,000–200,000 per
mammalian cell) was greatest in brain, followed by colon and heart, and then liver, lung, and
kidney. The majority of endogenous AP sites were cleaved 5′ to the AP site. These data
suggest that removal of the deoxyribosyl phosphate moiety is the rate-limiting step in base …
Abstract
Apurinic/apyrimidinic (AP) sites are one of the most frequent lesions in DNA. Using a highly sensitive slot blot assay, we determined the number and condition of endogenous AP sites in normal tissues of rats and human liver. The number of AP sites (50,000–200,000 per mammalian cell) was greatest in brain, followed by colon and heart, and then liver, lung, and kidney. The majority of endogenous AP sites were cleaved 5′ to the AP site. These data suggest that removal of the deoxyribosyl phosphate moiety is the rate-limiting step in base excision and AP site repair in vivo.
AACR