[HTML][HTML] Identification of neuropeptide FF-related peptides in human cerebrospinal fluid by mass spectrometry

O Burlet-Schiltz, H Mazarguil, JC Sol, P Chaynes… - FEBS letters, 2002 - Elsevier
O Burlet-Schiltz, H Mazarguil, JC Sol, P Chaynes, B Monsarrat, JM Zajac, A Roussin
FEBS letters, 2002Elsevier
Several neuropeptide FF (NPFF)-related peptides, known as modulators of the opioid
system, have been previously characterized in bovine and rodent brain. Reverse-phase
high pressure liquid chromatography (HPLC) fractions of a human with normal pressure
hydrocephalus cerebrospinal fluid (CSF), co-migrating with NPFF-related synthetic peptides,
were characterized by capillary HPLC coupled on-line to nanospray ion trap tandem mass
spectrometry. Two peptides present in the pro-NPFFA precursor, NPAF …
Several neuropeptide FF (NPFF)-related peptides, known as modulators of the opioid system, have been previously characterized in bovine and rodent brain. Reverse-phase high pressure liquid chromatography (HPLC) fractions of a human with normal pressure hydrocephalus cerebrospinal fluid (CSF), co-migrating with NPFF-related synthetic peptides, were characterized by capillary HPLC coupled on-line to nanospray ion trap tandem mass spectrometry. Two peptides present in the pro-NPFFA precursor, NPAF (AGEGLNSQFWSLAAPQRF-NH2) and NPSF (SLAAPQRF-NH2), were identified. The monitoring of NPFF-related peptides in human CSF can be helpful to understand their roles in pain sensitivity.
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