Identification of mannose 6-phosphate in two asparagine-linked sugar chains of recombinant transforming growth factor-beta 1 precursor.
AF Purchio, JA Cooper, AM Brunner, MN Lioubin… - Journal of Biological …, 1988 - ASBMB
Recombinant transforming growth factor-beta 1 (TGF-beta 1) precursor produced and
secreted by a clone of Chinese hamster ovary cells was found to be glycosylated and
phosphorylated. Treatment of 32P-labeled precursor protein with N-glycanase indicated that
phosphate was incorporated into asparagine-linked complex carbohydrate moieties.
Fractionation of 32P-labeled glycopeptides followed by amino acid sequence analysis
indicated that greater than 95% of the label was incorporated into two out of three …
secreted by a clone of Chinese hamster ovary cells was found to be glycosylated and
phosphorylated. Treatment of 32P-labeled precursor protein with N-glycanase indicated that
phosphate was incorporated into asparagine-linked complex carbohydrate moieties.
Fractionation of 32P-labeled glycopeptides followed by amino acid sequence analysis
indicated that greater than 95% of the label was incorporated into two out of three …